5SWF

The structure of the PP2A B56 subunit double phosphorylated BubR1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-09
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	53.307, 107.422, 117.719
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.500 - 2.818
R-factor	0.2099
Rwork	0.207
R-free	0.23050
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5k6s
RMSD bond length	0.002
RMSD bond angle	0.734
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.511	2.970
High resolution limit [Å]	2.818	2.820
Rmerge	0.075	0.894
Number of reflections	16683
<I/σ(I)>	13.5	1.8
Completeness [%]	98.7	93.8
Redundancy	5.3	5.3
CC(1/2)		0.686

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.75	298	8% PEG 8k, 0.8 M HEPES, 0.8 M LiCl