5S5Z
Tubulin-Z2856434944-complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-14 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.91587 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 105.180, 158.030, 182.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 91.080 - 2.550 |
R-factor | 0.2111 |
Rwork | 0.209 |
R-free | 0.25250 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 5lxt |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX (1.18.2_3874) |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 91.080 | 91.080 | 2.620 |
High resolution limit [Å] | 2.550 | 11.400 | 2.550 |
Rmerge | 0.122 | 0.031 | 2.389 |
Rmeas | 0.133 | 0.034 | 2.590 |
Total number of observations | 668786 | ||
Number of reflections | 98956 | 1234 | 7237 |
<I/σ(I)> | 9.64 | 44.6 | 0.75 |
Completeness [%] | 99.5 | 99 | 99.8 |
Redundancy | 6.758 | 6.262 | 6.711 |
CC(1/2) | 0.999 | 0.999 | 0.326 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine |