5S5W

Tubulin-Z53860899-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	P 21 21 21
Unit cell lengths	104.870, 158.350, 178.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.190 - 2.350
R-factor	0.2256
Rwork	0.224
R-free	0.26530
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	63.190	63.190	2.410
High resolution limit [Å]	2.350	10.510	2.350
Rmerge	0.161	0.031	2.530
Rmeas	0.174	0.033	2.729
Total number of observations	827544
Number of reflections	123973	1532	9112
<I/σ(I)>	9.46	46.25	0.72
Completeness [%]	99.7	98.9	99.9
Redundancy	6.675	6.294	7.061
CC(1/2)	0.998	0.999	0.207

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine