5OVU
Cupriavidus metallidurans BPH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-09-21 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1 |
| Spacegroup name | P 6 |
| Unit cell lengths | 96.496, 96.496, 75.894 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.900 - 2.100 |
| R-factor | 0.2377 |
| Rwork | 0.237 |
| R-free | 0.25227 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ovs |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.919 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.900 | 2.220 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.144 | 1.120 |
| Number of reflections | 23627 | 3757 |
| <I/σ(I)> | 11.7 | 2.03 |
| Completeness [%] | 99.8 | 99.3 |
| Redundancy | 9.39 | 9.33 |
| CC(1/2) | 0.997 | 0.796 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 100 mM sodium acetate pH 4.4, 1.5 M sodium nitrate |
