5OV3
Structure of the RbBP5 beta-propeller domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 77 |
Detector technology | PIXEL |
Collection date | 2015-08-14 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.809, 71.897, 178.269 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 66.678 - 2.450 |
R-factor | 0.1995 |
Rwork | 0.196 |
R-free | 0.27100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xl2 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.041 |
Data reduction software | XDS |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 71.900 |
High resolution limit [Å] | 2.450 |
Number of reflections | 27559 |
<I/σ(I)> | 12.7 |
Completeness [%] | 99.8 |
Redundancy | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 300 | 0.1M MES (pH 6.5), 40 % PEG 200 |