5OSA
GLIC-GABAAR alpha1 chimera crystallized at pH4.6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-07-07 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9795 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 185.017, 133.947, 162.697 |
Unit cell angles | 90.00, 103.53, 90.00 |
Refinement procedure
Resolution | 29.980 - 2.750 |
R-factor | 0.20463086704 |
Rwork | 0.203 |
R-free | 0.22886 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4hfi |
RMSD bond length | 0.005 |
RMSD bond angle | 0.813 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (dev_2645) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 95.280 | 2.800 |
High resolution limit [Å] | 2.750 | 2.750 |
Rmerge | 0.068 | 0.772 |
Rpim | 0.066 | 0.730 |
Number of reflections | 100157 | 4988 |
<I/σ(I)> | 1.2 | 1.2 |
Completeness [%] | 99.5 | 99.9 |
Redundancy | 3.5 | 3.5 |
CC(1/2) | 0.946 | 0.868 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 289.15 | 100 mM NaCl, 100 mM sodium acetate pH 4.6, 12 % PEG 6000 |