5OQA
XFEL structure of the off state of a reversibly photoswitching fluorescent protein determined using the GDVN injection method
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | FREE ELECTRON LASER |
Source details | SLAC LCLS BEAMLINE XPP |
Synchrotron site | SLAC LCLS |
Beamline | XPP |
Temperature [K] | 293 |
Detector technology | PIXEL |
Collection date | 2016-06-17 |
Detector | CS-PAD XPP |
Wavelength(s) | 1.308 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 39.800, 74.600, 79.200 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 54.300 - 1.530 |
R-factor | 0.17904 |
Rwork | 0.176 |
R-free | 0.22831 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.018 |
RMSD bond angle | 1.999 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 54.300 |
High resolution limit [Å] | 1.530 |
Number of reflections | 36135 |
<I/σ(I)> | 5.96 |
Completeness [%] | 78.3 |
Redundancy | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 293 | 0.2 M lithium sulfate, 0.1 M Tris-Cl pH 8.5, 23-30% PEG 3350 |