5OOW
Crystal structure of lobe II from the nucleotide binding domain of DnaK in complex with AMPPCP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-16 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.00003 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 59.857, 64.203, 74.639 |
Unit cell angles | 90.00, 113.31, 90.00 |
Refinement procedure
Resolution | 54.740 - 2.900 |
R-factor | 0.239 |
Rwork | 0.239 |
R-free | 0.24900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4jn4 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.900 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.740 | 3.080 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.255 | 0.966 |
Rpim | 0.157 | 0.597 |
Number of reflections | 11517 | 1846 |
<I/σ(I)> | 7.7 | |
Completeness [%] | 98.7 | 99.4 |
Redundancy | 6.9 | 6.7 |
CC(1/2) | 0.979 | 0.730 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 25% PEG1500, 0.1 M MIB buffer pH 7.0 |