5O6U
Structure of the Cascade-I-Fv R-loop complex from Shewanella putrefaciens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-13 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.991 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 143.316, 143.316, 172.698 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.911 - 3.250 |
| R-factor | 0.254 |
| Rwork | 0.252 |
| R-free | 0.28560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.827 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.660 | 3.360 |
| High resolution limit [Å] | 3.250 | 3.250 |
| Rmerge | 0.168 | |
| Number of reflections | 32784 | |
| <I/σ(I)> | 11.66 | 1.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.1 | 11.4 |
| CC(1/2) | 0.990 | 0.850 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 22.5 % w/v PEG4000, 15 % v/v glycerol, 153 mM ammonium acetate and 85 mM sodium citrate pH 5.6 |
