5O29
Lytic transglycosylase in action
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-07-20 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.218, 72.749, 125.225 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.930 - 1.379 |
R-factor | 0.1836 |
Rwork | 0.183 |
R-free | 0.21300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4YIM |
RMSD bond length | 0.005 |
RMSD bond angle | 0.768 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.930 | 1.428 |
High resolution limit [Å] | 1.379 | 1.379 |
Rmerge | 0.143 | |
Rpim | 0.070 | 0.100 |
Number of reflections | 119443 | 11548 |
<I/σ(I)> | 10.73 | 0.4 |
Completeness [%] | 93.8 | 92.1 |
Redundancy | 4.7 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277.15 | 33% (w/v) PEG 6000, Hepes pH 7.5 or 3) 0.2M Zinc acetate, or 0.1 M Sodium cacodylate pH 6.5 |