5NTU
Crystal Structure of human Pro-myostatin Precursor at 2.6 A Resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-01-16 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97625 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 168.157, 36.301, 120.448 |
Unit cell angles | 90.00, 104.39, 90.00 |
Refinement procedure
Resolution | 28.270 - 2.580 |
R-factor | 0.217 |
Rwork | 0.215 |
R-free | 0.26000 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 0.550 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 76.264 | 76.264 | 2.620 |
High resolution limit [Å] | 2.575 | 6.989 | 2.575 |
Rmeas | 0.071 | 0.046 | 1.037 |
Rpim | 0.034 | 0.022 | 0.493 |
Total number of observations | 90386 | ||
Number of reflections | 22474 | ||
<I/σ(I)> | 11.7 | ||
Completeness [%] | 97.7 | 99.1 | 99.6 |
Redundancy | 4 | 3.6 | 4.2 |
CC(1/2) | 0.997 | 0.997 | 0.583 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 292 | 10% PEG6000, 0.1M HEPES: cryo-protection: 70% ethylene glycol |