5NRU
Cys-Gly dipeptidase GliJ in complex with Zn2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-19 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.28096 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 99.210, 99.210, 106.670 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 2.150 |
| R-factor | 0.19487 |
| Rwork | 0.194 |
| R-free | 0.21076 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5lx0 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.050 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.250 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.080 | 0.556 |
| Number of reflections | 63700 | |
| <I/σ(I)> | 9.6 | 2.3 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.1 M MES pH 6.0, 5% isopropanol |
