5NN2
The structure of the polo-box domain (PBD) of Plk1 in complex with Z228588490
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-15 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.360, 93.380, 35.900 |
| Unit cell angles | 90.00, 100.72, 90.00 |
Refinement procedure
| Resolution | 35.273 - 1.810 |
| R-factor | 0.1887 |
| Rwork | 0.187 |
| R-free | 0.21860 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.987 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.273 | 1.860 |
| High resolution limit [Å] | 1.810 | 1.810 |
| Rmerge | 0.053 | 0.908 |
| Rpim | 0.042 | 0.599 |
| Number of reflections | 19250 | 1419 |
| <I/σ(I)> | 11.2 | 1.5 |
| Completeness [%] | 97.8 | 96.8 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 200 mM K/Na Tartrate, 10-20% PEG3350 |
