5NMP
Isatin hydrolase A (IHA) from Ralstonia solanacearum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-1 |
Synchrotron site | ESRF |
Beamline | MASSIF-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-11-01 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.9762 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 164.840, 164.840, 275.890 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 99.199 - 2.650 |
R-factor | 0.1625 |
Rwork | 0.161 |
R-free | 0.19250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4j0n |
RMSD bond length | 0.008 |
RMSD bond angle | 0.653 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.199 | 2.720 |
High resolution limit [Å] | 2.650 | 2.650 |
Rmerge | 0.093 | |
Rpim | 0.057 | 0.663 |
Number of reflections | 125897 | 9226 |
<I/σ(I)> | 15.6 | 1.5 |
Completeness [%] | 95.1 | 99.4 |
Redundancy | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 1 M succinate, 0.1 M HEPES |