5NB4
Atomic resolution structure of C-phycoerythrin from marine cyanobacterium Phormidium sp. A09DM at pH 7.5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-09-14 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97951 |
Spacegroup name | P 1 |
Unit cell lengths | 109.046, 109.095, 117.371 |
Unit cell angles | 78.78, 82.32, 60.26 |
Refinement procedure
Resolution | 94.620 - 1.140 |
R-factor | 0.149 |
Rwork | 0.147 |
R-free | 0.18400 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 5aqd |
RMSD bond length | 0.017 |
RMSD bond angle | 2.682 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 94.620 | 1.160 |
High resolution limit [Å] | 1.140 | 1.140 |
Rmerge | 0.042 | 0.545 |
Rpim | 0.042 | 0.545 |
Number of reflections | 1575226 | 74960 |
<I/σ(I)> | 10.7 | 1.6 |
Completeness [%] | 93.8 | 90 |
Redundancy | 2.6 | 2.7 |
CC(1/2) | 0.998 | 0.601 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294.15 | Morpheus screen condition C8 with the NPS mix of additives |