5N6P

AMPA receptor NTD mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-29
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9393
Spacegroup name	P 41 21 2
Unit cell lengths	94.500, 94.500, 160.570
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.710 - 2.800
R-factor	0.20817
Rwork	0.206
R-free	0.24933
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3hsy
RMSD bond length	0.016
RMSD bond angle	1.750
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.710	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.119	0.846
Rpim	0.069	0.483
Number of reflections	18494	2683
<I/σ(I)>	9.4	1.9
Completeness [%]	99.6	100
Redundancy	3.9	4
CC(1/2)	0.993	0.498

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	291	16-20% PEG3350, 200 mM sodium formate