5N2V
Changes in conformational equilibria regulate the activity of the Dcp2 decapping enzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-02 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.99 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.841, 76.522, 89.526 |
| Unit cell angles | 90.00, 102.83, 90.00 |
Refinement procedure
| Resolution | 48.289 - 3.100 |
| R-factor | 0.2414 |
| Rwork | 0.239 |
| R-free | 0.28680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5j3t |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.487 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.300 | 3.270 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.240 | 0.730 |
| Number of reflections | 15440 | |
| <I/σ(I)> | 4.3 | 1.8 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.4 | 3.4 |
| CC(1/2) | 0.950 | 0.640 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 41% M1K3350 Morpheus Mix 4, MOPS pH 7.0, 0.06 M MgCl2/CaCl2 |
