5MVI
Crystal structure of 2-methylcitrate dehydratase (PrpD) from Salmonella enterica
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-05-01 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.9282 |
Spacegroup name | H 3 |
Unit cell lengths | 139.662, 139.662, 513.484 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.140 - 3.050 |
R-factor | 0.21437 |
Rwork | 0.213 |
R-free | 0.23870 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.419 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 39.140 |
High resolution limit [Å] | 3.050 |
Number of reflections | 70843 |
<I/σ(I)> | 5.7 |
Completeness [%] | 99.5 |
Redundancy | 9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.2M Sodium fluoride 20% w/vPEG 3350 |