5MVF
Active structure of EHD4 complexed with ADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-06-10 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.918409 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 199.469, 199.469, 41.790 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.378 - 3.268 |
R-factor | 0.239 |
Rwork | 0.238 |
R-free | 0.26440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4cid |
RMSD bond length | 0.002 |
RMSD bond angle | 0.563 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.380 | 3.460 |
High resolution limit [Å] | 3.268 | 3.270 |
Rmerge | 0.274 | |
Rmeas | 2.115 | |
Rpim | 0.088 | |
Number of reflections | 13647 | |
<I/σ(I)> | 9.53 | 1.02 |
Completeness [%] | 99.5 | 97 |
Redundancy | 10.5 | |
CC(1/2) | 0.995 | 0.361 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 900 mM sodium malonate (pH 7) |