5MV8

Structure of human Myosin 7b C-terminal MyTH4-FERM domain in complex with harmonin-a PDZ3 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.906032
Spacegroup name	P 1 21 1
Unit cell lengths	69.620, 42.560, 118.170
Unit cell angles	90.00, 98.02, 90.00

Refinement procedure

Resolution	35.330 - 1.880
R-factor	0.191
Rwork	0.190
R-free	0.21900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	0.990
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.880
Number of reflections	53567
<I/σ(I)>	12.77	1
Completeness [%]	95.0	61
Redundancy	6.4
CC(1/2)	0.998	0.543

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	290	7% (w/v) PEG 4000, 0.1 M HEPES pH 7.5, 50 mM MgCl2