5MTJ
Yes1-SH2 in complex with monobody Mb(Yes_1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-02-20 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.97919 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 101.864, 101.864, 139.618 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.308 - 1.949 |
R-factor | 0.1918 |
Rwork | 0.190 |
R-free | 0.22310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3K2M and 4TZI |
RMSD bond length | 0.008 |
RMSD bond angle | 0.872 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.980 |
High resolution limit [Å] | 1.949 | 1.950 |
Rmerge | 0.127 | |
Rmeas | 0.131 | |
Rpim | 0.040 | 0.544 |
Number of reflections | 27106 | |
<I/σ(I)> | 24.44 | 1.5 |
Completeness [%] | 99.8 | 99.7 |
Redundancy | 19.1 | 16.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 10.25 | 291 | 2M Ammonium sulfate, 0.2M Lithium sulfate, 0.1M CAPS/NaOH pH10.25 |