5MR1

Crystal structure of the Pleckstrin homology domain of Interactor protein for cytohesin exchange factors 1 (IPCEF1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-12
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.92
Spacegroup name	P 21 21 21
Unit cell lengths	43.974, 49.612, 53.027
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.900 - 1.200
R-factor	0.1677
Rwork	0.167
R-free	0.18760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1upq
RMSD bond length	0.011
RMSD bond angle	1.091
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.960	1.220
High resolution limit [Å]	1.200	1.200
Rmerge	0.057	1.593
Number of reflections	35945
<I/σ(I)>	19.6	1.4
Completeness [%]	97.2	90.7
Redundancy	13.4	10.5
CC(1/2)	0.999	0.551

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	0.2 M Lithium Sulfate, 25% PEG 3350, 0.1 M Bis Tris pH 5.5