5MK2
Crystal structure of the His Domain Protein Tyrosine Phosphatase (HD-PTP/PTPN23) Bro1 domain (CHMP4B peptide complex structure)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-12-17 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 68.440, 64.860, 81.230 |
Unit cell angles | 90.00, 90.19, 90.00 |
Refinement procedure
Resolution | 50.685 - 1.700 |
R-factor | 0.1708 |
Rwork | 0.169 |
R-free | 0.20320 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3rau |
RMSD bond length | 0.006 |
RMSD bond angle | 0.748 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11_2563: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.685 |
High resolution limit [Å] | 1.700 |
Rmerge | 0.041 |
Number of reflections | 77355 |
<I/σ(I)> | 18.56 |
Completeness [%] | 98.7 |
Redundancy | 3.3 |
CC(1/2) | 0.998 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 277 | 0.2 M CaCl2, 0.1 M MES pH 6.0, 20% PEG 6K |