5MFF
Designed armadillo repeat protein YIIIM5AII in complex with peptide (RR)5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-10-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 81.755, 90.886, 179.659 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.310 - 1.900 |
R-factor | 0.186 |
Rwork | 0.184 |
R-free | 0.21300 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.110 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.310 | 1.950 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.108 | 4.680 |
Number of reflections | 106118 | |
<I/σ(I)> | 14.51 | 0.7 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 11 | 11.2 |
CC(1/2) | 0.999 | 0.270 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 60% (NH4)2 SO4, 0.1M HEPES pH 8.0 |