5M8Q
Crystal structure of human tyrosinase related protein 1 mutant (T391V-R374S-Y362F) in complex with kojic acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-1 |
Synchrotron site | ESRF |
Beamline | MASSIF-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-10-01 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.976 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 89.640, 140.426, 191.559 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.875 - 2.850 |
R-factor | 0.2114 |
Rwork | 0.208 |
R-free | 0.27540 |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | REFMAC |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 48.770 |
High resolution limit [Å] | 2.850 |
Number of reflections | 57320 |
<I/σ(I)> | 9.9 |
Completeness [%] | 100.0 |
Redundancy | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 290 | 0.1 M Tris (pH 7.0), 0.2 M NaCl and 30% (w/v) PEG 3000 |