5M8K
Crystal structure of Eremococcus coleocola manganese transporter mutant E129Q
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2016-04-07 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 151.260, 81.770, 96.500 |
Unit cell angles | 90.00, 107.60, 90.00 |
Refinement procedure
Resolution | 11.997 - 3.600 |
R-factor | 0.2234 |
Rwork | 0.221 |
R-free | 0.26520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5m87 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.494 |
Data reduction software | XDS |
Data scaling software | XDS |
Refinement software | PHENIX ((1.11_2567: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.700 |
High resolution limit [Å] | 3.600 | 3.600 |
Rmeas | 0.069 | 1.658 |
Number of reflections | 13025 | |
<I/σ(I)> | 12.8 | 1.78 |
Completeness [%] | 98.7 | 99.8 |
Redundancy | 6.7 | |
CC(1/2) | 0.998 | 0.817 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 50 mM Tris-HCl pH 8.6 22.6% PEG 400 (v/v) |