5LYU
The native crystal structure of 7SK 5'-hairpin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9797 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.790, 47.780, 68.670 |
| Unit cell angles | 90.00, 99.05, 90.00 |
Refinement procedure
| Resolution | 28.320 - 2.200 |
| R-factor | 0.1935 |
| Rwork | 0.191 |
| R-free | 0.23960 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 5lys |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.810 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER-TNT (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.330 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.039 | 0.939 |
| Number of reflections | 13144 | |
| <I/σ(I)> | 18.57 | 0.97 |
| Completeness [%] | 87.3 | 52 |
| Redundancy | 3.4 | 2.38 |
| CC(1/2) | 0.999 | 0.425 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 30% PEG 1000, 50 mM Tris pH 7.5, 50 to 75 mM NaCl, 50 mM MgCl2 |
