5LO7
Crystal structure of self-complemented MyfA, the major subunit of Myf fimbriae from Yersinia enterocolitica
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-09-09 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9737 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.980, 41.970, 150.830 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.681 - 1.900 |
R-factor | 0.1623 |
Rwork | 0.159 |
R-free | 0.19120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5lnd |
RMSD bond length | 0.012 |
RMSD bond angle | 1.431 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 150.830 | 29.681 | 1.900 |
High resolution limit [Å] | 1.800 | 5.690 | 1.800 |
Rmerge | 0.032 | 0.758 | |
Rmeas | 0.099 | 0.038 | 0.885 |
Rpim | 0.049 | 0.019 | 0.447 |
Total number of observations | 94487 | 3071 | 13603 |
Number of reflections | 25547 | ||
<I/σ(I)> | 9.6 | 20.4 | 1.8 |
Completeness [%] | 99.9 | 97.4 | 100 |
Redundancy | 3.7 | 3.4 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 294 | 35% dioxane v/v |