5LJC
Crystal structure of holo human CRBP1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-03-02 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.91881 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 34.035, 48.563, 75.748 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.883 - 1.433 |
R-factor | 0.181 |
Rwork | 0.179 |
R-free | 0.21380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ljb |
RMSD bond length | 0.012 |
RMSD bond angle | 1.713 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.883 | 1.510 |
High resolution limit [Å] | 1.430 | 1.430 |
Rmerge | 0.063 | 0.504 |
Number of reflections | 23562 | |
<I/σ(I)> | 9.2 | |
Completeness [%] | 94.4 | 82.5 |
Redundancy | 3.3 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5 | 279 | 0.2 M ammonium chloride, 0.1 M sodium acetate, 0.2 mM zinc chloride, pH 5,0, 20% PEG 6000 |