5LFL
MamA RS-1 ArsTM double mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-02-27 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.939 |
Spacegroup name | I 4 |
Unit cell lengths | 150.548, 150.548, 204.297 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.920 - 3.370 |
R-factor | 0.1972 |
Rwork | 0.196 |
R-free | 0.21397 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.530 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | BALBES |
Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 47.920 |
High resolution limit [Å] | 3.370 |
Number of reflections | 30293 |
<I/σ(I)> | 24.09 |
Completeness [%] | 100.0 |
Redundancy | 8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 2.8 M sodium acetate |