5L6Q
Refolded AL protein from cardiac amyloidosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-01 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 58.020, 105.810, 34.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.400 |
| R-factor | 0.14904 |
| Rwork | 0.147 |
| R-free | 0.18208 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4od2 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.668 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.500 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.054 | 0.525 |
| Number of reflections | 42100 | |
| <I/σ(I)> | 12.6 | 2.6 |
| Completeness [%] | 97.6 | 98.7 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M MES, 18% PEG 8000, 0.1 M Zinc acetate |
