5KXV
Structure Proteinase K at 0.98 Angstroms
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2015-04-12 |
Detector | RIGAKU RAXIS V |
Wavelength(s) | 0.8 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 67.577, 67.577, 107.140 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 16.894 - 0.980 |
R-factor | 0.1009 |
Rwork | 0.100 |
R-free | 0.11400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4b5l |
RMSD bond length | 0.006 |
RMSD bond angle | 0.967 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.000 |
High resolution limit [Å] | 0.980 | 0.980 |
Rmerge | 0.076 | 0.489 |
Number of reflections | 141433 | |
<I/σ(I)> | 58.41 | 6.47 |
Completeness [%] | 99.5 | 99.2 |
Redundancy | 10.7 | 10.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 6.5 | 293 | 250 mM NaNO3, 50 mM CaCl2, 50 mM MES-NaOH |