5KUX

Designed influenza hemagglutinin binding protein HSB.2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-01-08
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9184
Spacegroup name	P 32 2 1
Unit cell lengths	58.385, 58.385, 69.449
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.877 - 1.800
R-factor	0.1981
Rwork	0.196
R-free	0.23610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2w9p
RMSD bond length	0.009
RMSD bond angle	0.955
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge		0.351
Number of reflections	12579
<I/σ(I)>	25.9	1.9
Completeness [%]	97.1	80.7
Redundancy	7.9	4.1
CC(1/2)		0.965

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293.15	15% PEG 8000, 0.1 M HEPES pH 7.9