5KN9

MutY N-terminal domain in complex with DNA containing an intrahelical oxoG:A base-pair

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-25
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	49.878, 38.759, 82.960
Unit cell angles	90.00, 105.66, 90.00

Refinement procedure

Resolution	79.879 - 1.930
R-factor	0.1718
Rwork	0.169
R-free	0.22870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rrq
RMSD bond length	0.008
RMSD bond angle	1.316
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	79.880	1.980
High resolution limit [Å]	1.930	1.930
Rmerge		0.110
Number of reflections	23193
<I/σ(I)>	10.9
Completeness [%]	99.5
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	300 mM Ca(OAc)2, 14% PEG 4000