5KL5
Wilms Tumor Protein (WT1) ZnF2-4 Q369H in complex with carboxylated DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-02-17 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 67.493, 77.211, 35.794 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.263 - 2.289 |
R-factor | 0.1957 |
Rwork | 0.193 |
R-free | 0.24630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4r2r |
RMSD bond length | 0.004 |
RMSD bond angle | 0.659 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((dev_2400: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.370 |
High resolution limit [Å] | 2.289 | 2.289 |
Rmerge | 0.113 | 0.480 |
Number of reflections | 8861 | |
<I/σ(I)> | 19.9 | 2.76 |
Completeness [%] | 99.5 | 95.6 |
Redundancy | 10.6 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 20% (w/v) polyethylene glycol 3350, 0.2 M ammonium acetate, and 0.1 M bistris-HCl, pH 6.5 |