5KJZ
Co-crystal structure of PKA RI alpha CNB-B mutant (G316R/A336T) with cGMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2012-10-21 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 29.461, 67.780, 77.960 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.264 - 1.347 |
R-factor | 0.1537 |
Rwork | 0.152 |
R-free | 0.18180 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.098 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.370 |
High resolution limit [Å] | 1.347 | 1.350 |
Rmerge | 0.062 | 0.214 |
Number of reflections | 34711 | |
<I/σ(I)> | 22.01 | 6.7 |
Completeness [%] | 98.6 | 83.6 |
Redundancy | 9.1 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 277.15 | 0.04 M critic acid, 0.06 M Bis-Tris propane, 24% w/v PEG 1500, 20% v/v glycerol |