5KJD
Synechocystis apocarotenoid oxygenase (ACO) mutant - Glu150Gln
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-04-23 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97919 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 118.530, 125.500, 203.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.420 - 2.750 |
R-factor | 0.21338 |
Rwork | 0.212 |
R-free | 0.24670 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ou9 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.268 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.420 | |
High resolution limit [Å] | 2.750 | 2.750 |
Number of reflections | 75683 | |
<I/σ(I)> | 7.6 | |
Completeness [%] | 95.0 | |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 281 | BisTris Propane, Sodium Polyacrylate 2100, Sodium Chloride |