5KJ4
Crystal Structure of Mouse Protocadherin-15 EC9-10
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-12-02 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97918 |
Spacegroup name | P 31 |
Unit cell lengths | 143.541, 143.541, 95.599 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.980 - 3.350 |
R-factor | 0.15943 |
Rwork | 0.158 |
R-free | 0.19590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4xhz |
RMSD bond length | 0.010 |
RMSD bond angle | 1.078 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.410 |
High resolution limit [Å] | 3.350 | 3.350 |
Rmerge | 0.256 | 0.931 |
Number of reflections | 31590 | |
<I/σ(I)> | 6.96 | 2.03 |
Completeness [%] | 100.0 | 100 |
Redundancy | 5.9 | 5.8 |
CC(1/2) | 0.910 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277.15 | 0.095M HEPES (pH7.5), 0.19M CaCl2, 26.6% v/v PEG 400 |