5KFK
Human DNA polymerase eta-DNA ternary complex: reaction with 10 mM Mn2+ for 300s
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-07-03 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 61 |
Unit cell lengths | 98.270, 98.270, 82.010 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.932 - 1.700 |
R-factor | 0.1749 |
Rwork | 0.172 |
R-free | 0.21020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ecq |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.740 | |
High resolution limit [Å] | 1.700 | 7.600 | 1.700 |
Rmerge | 0.067 | 0.015 | 0.698 |
Number of reflections | 49456 | ||
<I/σ(I)> | 24.82 | 103.7 | 3.02 |
Completeness [%] | 99.9 | 94.1 | 100 |
Redundancy | 7.5 | ||
CC(1/2) | 0.999 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 6 | 298 | 17% PEG 2000MME, 0.1 M MES |