5KDN
ZmpB metallopeptidase from Clostridium perfringens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-28 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.890, 68.020, 170.860 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.090 - 1.660 |
| R-factor | 0.15508 |
| Rwork | 0.154 |
| R-free | 0.18046 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5kdj |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.863 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.090 | 1.680 |
| High resolution limit [Å] | 1.660 | 1.660 |
| Rmerge | 0.054 | 0.249 |
| Number of reflections | 92133 | |
| <I/σ(I)> | 18.5 | 5.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.2 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | PEG 3350, Na/K tartrate, HEPES pH 7.5 |
