5K45
Wolinella succinogenes L-asparaginase P121 + L-Glutamic acid
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-06-30 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 145.806, 70.961, 142.276 |
Unit cell angles | 90.00, 118.08, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.630 |
R-factor | 0.1643 |
Rwork | 0.163 |
R-free | 0.19750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wsa |
RMSD bond length | 0.018 |
RMSD bond angle | 1.748 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 29.330 | 1.720 |
High resolution limit [Å] | 1.620 | 4.810 | 1.620 |
Rmerge | 0.081 | 0.039 | 0.661 |
Number of reflections | 155380 | ||
<I/σ(I)> | 9.09 | 21.8 | 1.7 |
Completeness [%] | 96.1 | 98.7 | 82.5 |
Redundancy | 3.71 | ||
CC(1/2) | 0.996 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 283 | PEG MME 2000 + 0.1M HEPES pH 7.5 |