5JUB
Crystal structure of ComR from S.thermophilus in complex with DNA and its signalling peptide ComS.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-11 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.978563 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 186.110, 46.190, 100.270 |
| Unit cell angles | 90.00, 98.23, 90.00 |
Refinement procedure
| Resolution | 46.553 - 2.570 |
| R-factor | 0.2288 |
| Rwork | 0.227 |
| R-free | 0.26790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | ComR apo |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.420 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.553 | 2.630 |
| High resolution limit [Å] | 2.570 | 2.570 |
| Rmerge | 0.086 | 0.580 |
| Number of reflections | 27134 | |
| <I/σ(I)> | 13.31 | 1.81 |
| Completeness [%] | 99.0 | 98.6 |
| Redundancy | 5.3 | |
| CC(1/2) | 0.990 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 290.15 | 30% PEG 550MME, 0.1M MgCl2, 0,05M Tris-HCl, pH=8.5 |
