5JRG
Crystal structure of the nucleosome containing the DNA with tetrahydrofuran (THF)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-12-15 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.98000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 99.491, 108.773, 169.571 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.146 - 2.500 |
R-factor | 0.1956 |
Rwork | 0.193 |
R-free | 0.24540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3lz0 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.279 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.590 |
High resolution limit [Å] | 2.500 | 5.380 | 2.500 |
Rmerge | 0.093 | 0.060 | 0.418 |
Number of reflections | 64545 | ||
<I/σ(I)> | 6.5 | ||
Completeness [%] | 99.8 | 99.8 | 99.8 |
Redundancy | 9.2 | 11.6 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | potassium cacodylate, potassium chloride, manganese chloride |