5JQA
CaM:RM20 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-11-24 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 29.016, 57.048, 45.068 |
Unit cell angles | 90.00, 98.17, 90.00 |
Refinement procedure
Resolution | 28.720 - 1.800 |
R-factor | 0.15515 |
Rwork | 0.153 |
R-free | 0.18845 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2o5g |
RMSD bond length | 0.010 |
RMSD bond angle | 1.357 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | |
High resolution limit [Å] | 1.720 | 1.720 |
Number of reflections | 15660 | |
<I/σ(I)> | 14.1 | |
Completeness [%] | 95.5 | |
Redundancy | 96.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.1 M sodium acetate, pH 4.6, 25% w/v PEG4000 |