5JO4
Antibody Fab Fragment Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-16 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.5 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 143.160, 50.780, 139.740 |
| Unit cell angles | 90.00, 90.90, 90.00 |
Refinement procedure
| Resolution | 50.390 - 2.530 |
| R-factor | 0.1997 |
| Rwork | 0.197 |
| R-free | 0.24780 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.359 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (dev_2037) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.390 |
| High resolution limit [Å] | 2.530 |
| Number of reflections | 33988 |
| <I/σ(I)> | 14.35 |
| Completeness [%] | 97.0 |
| Redundancy | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M NH4SO4, 12% PEG 3350 |
