5JHF
Crystal structure of Atg13(17BR)-Atg13(17LR)-Atg17-Atg29-Atg31 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2013-11-21 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 147.956, 64.043, 184.387 |
Unit cell angles | 90.00, 109.91, 90.00 |
Refinement procedure
Resolution | 49.220 - 3.210 |
R-factor | 0.22596 |
Rwork | 0.224 |
R-free | 0.26510 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4p1w |
RMSD bond length | 0.014 |
RMSD bond angle | 1.726 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.260 |
High resolution limit [Å] | 3.200 | 3.200 |
Number of reflections | 53805 | |
<I/σ(I)> | 16.3 | |
Completeness [%] | 99.9 | |
Redundancy | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 5% polyethylene glycol monomethyl ether 5000, 100 mM Tris-HCl |