5JFZ
E. coli EcFicT in complex with EcFicA mutant E28G
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-10-31 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 154.778, 64.402, 87.884 |
| Unit cell angles | 90.00, 113.80, 90.00 |
Refinement procedure
| Resolution | 80.410 - 2.400 |
| R-factor | 0.2244 |
| Rwork | 0.222 |
| R-free | 0.27270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5fjj |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.528 |
| Data scaling software | Aimless (0.1.27) |
| Phasing software | PHASER (2.5.1) |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 80.410 | 80.410 | 2.490 |
| High resolution limit [Å] | 2.400 | 8.970 | 2.400 |
| Rmerge | 0.176 | 0.068 | 0.651 |
| Rmeas | 0.193 | 0.074 | 0.710 |
| Rpim | 0.076 | 0.030 | 0.277 |
| Total number of observations | 192193 | 3665 | 17547 |
| Number of reflections | 30850 | ||
| <I/σ(I)> | 7.2 | 16.1 | 2.3 |
| Completeness [%] | 98.6 | 99.9 | 86.6 |
| Redundancy | 6.2 | 5.7 | 6.2 |
| CC(1/2) | 0.992 | 0.993 | 0.774 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293.15 | 19% PEG 1500 (w/v), 0.1 M MMT (malic acid, MES, Tris) buffer pH 6.0 |
