5JED
Apo-structure of humanised RadA-mutant humRadA28
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-02-13 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.96862 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 37.771, 72.100, 41.927 |
Unit cell angles | 90.00, 115.89, 90.00 |
Refinement procedure
Resolution | 37.720 - 1.332 |
R-factor | 0.13669 |
Rwork | 0.135 |
R-free | 0.17122 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5j4l |
RMSD bond length | 0.012 |
RMSD bond angle | 1.579 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.720 | 1.337 |
High resolution limit [Å] | 1.332 | 1.332 |
Rmerge | 0.093 | 1.086 |
Number of reflections | 45801 | |
<I/σ(I)> | 7.6 | 2.1 |
Completeness [%] | 99.3 | 99.1 |
Redundancy | 4.7 | 3.2 |
CC(1/2) | 0.992 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | 0.1M NaCacodylate pH=6.5, 5% PEG8000, 40% MPD |