5J9O

tRNA guanine Transglycosylase (TGT) in co-crystallized complex with 1-(2-(methylamino)-1H-benzo[d]imidazol-6-yl)guanidine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-08-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.918409
Spacegroup name	C 1 2 1
Unit cell lengths	90.102, 63.798, 70.955
Unit cell angles	90.00, 92.95, 90.00

Refinement procedure

Resolution	19.450 - 1.410
R-factor	0.1414
Rwork	0.140
R-free	0.16930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1p0d
RMSD bond length	0.005
RMSD bond angle	0.776
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1-2155_1492))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.450	1.490
High resolution limit [Å]	1.410	1.410
Rmerge		0.493
Number of reflections	76131
<I/σ(I)>	16.88	2.26
Completeness [%]	97.9	95.6
Redundancy	3.8	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	288	13% PEG 8000,100mM MES, 1mM DTT, 10% DMSO,