5J5J
Crystal structure of a chimera of human Desmocollin-2 EC1 and human Desmoglein-2 EC2-EC5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-06-11 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 63.494, 87.489, 218.536 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 68.295 | 3.550 |
High resolution limit [Å] | 3.290 | 3.300 |
Rmerge | 1.577 | |
Number of reflections | 19019 | |
<I/σ(I)> | 10.1 | 1.1 |
Completeness [%] | 99.4 | 98.5 |
Redundancy | 5.8 | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 33% (w/v) Ethylene glycol-PEG 8000, 0.1M Tris-bicine pH 8.5, 10%(v/v) monosaccharides mix (Molecular Dimensions), 0.01M calcium chloride |